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    V5-Tag Monoclonal Antibody

    货号:PGM3005
    包装:100μg
    价格:1500
    储存:-20°C

    详细描述

    Catalog No.
    PGM3005
    Size
    100ug
    Source
    Mouse
    Immunogen
    Synthetic Peptide
    Purification
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    Specificity
    The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.
    Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
    Concentration
    1 mg/ml
    Storage / Stability
    -20°C/1 year
    Reactivity
    N/A
    Applications
    WB, IP, IF
    Dilution
    WB: 1:5000 IP: 1:200 IF: 1:1000
    V5-Tag Monoclonal Antibody Images
    IP antibody use: 5ug V5 Mouse IgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with V5-tag fusion protein 3) IP (untransfted 293+anti-V5 mAb+Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG+Prote
    IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)
    1ug V5 fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
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